Huperzia serrata Thunb. is a valuable medicinal plant containing alcaloid huperzine - a major active compound in the treatment of Alzheimer’s disease in the elderly. The aim of this study is to propagate H. serrata using the in vitro methods. The samples (crown buds without branching) were sterilised by immersing in the solution of NaOCl 3% in 30 minutes, then treating with 30% H2 O2 solution for 7 minutes. The results showed that using the MS2 medium (including MS + 0.3 mg/l BAP + 0.01 mg/l IBA) supplemented with a mixture of antimicrobial substances that consisted of 0.5 mg/l malachite green and 100 ml/l antibiotic AAS (including 30 mg/l penicillin, 50 mg/l streptomycin and 125 μg/l amphotericin B) gave the highest rate of the successful samples at 56.67% after 30 days of cultivation and shoots began to branch. The initial successful samples continued being transplanted into the MS1 medium, and then the branching rate and the rate of rooting reached 34.11% and 19.67%, respectively after 60 days of cultivation. After 6 months, shoots grew by 3.48 times and the rate of rooting was up to 53.12%.